Team:ESBS-Strasbourg/Concentrations Measurements

Revision as of 13:06, 29 July 2008 by Paulo (Talk | contribs)

1)by spectrophotometer

step1: Prepare your sample:

2µL of the solution 

400µL Water

step2: put lambda=260nm

step3: do the blank with the same water you used for dilution

step4: Do the conversion absorbance->concentrations

1DO = 50µgDNA/mL = 50ng/µL

=>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL.

By nanodrop

Ask Maria to use it at the Pharma faculty. You only need 1µL of your solution.