User:University of Washington/29 July 2008

From 2008.igem.org

Revision as of 23:57, 29 July 2008 by Funfai (Talk | contribs)

luxR from pLac

-Bacterial stab of part I0462 received from iGEM HQ.

-Sequence of R0010+E0240 ligation received back and found to be incorrect. Assembly of these parts must be retried.

-Restriction digests of R0010 and E0240 started; will incubate at 37 overnight.

LuxR from AraC and TetR

-PCR purified the AraC and TetR promoter.

-Digest the Elowitz's plasmid with enzyme HindIII to find out what plasmid exactly the strain contain. Two potential >>(pCS26 has 4 sites: expected 4716, 1990, 1982, 785 bp) (pACYC184 has 1 site: 4245 bp)

  • 34.5ul ddH2O + 5ul NEB2 + 10ul DNA(assume 100 ng/ul) + ...vortex... + 0.5 ul HindIII +....centrifuge ...
  • incubated 37 degree Celsius for 1.5 hours

-Ran gel.



Back to Team:University_of_Washington/Notebook#Notebook