Team:University of Ottawa/19 July 2008

From 2008.igem.org

Revision as of 18:30, 30 July 2008 by Ttye065 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Untitled Document

 

 


Contents

Today in the lab

Dan

Ligation
  • Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
    • Ligation of Atcre
  • Began ligation of Chris's Atcre component in ~50 uL reaction

    • Matt

    Inoculation
  • Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
  • I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
    • PCR
  • I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/19_July_2008"