User:University of Washington/30 July 2008

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Lambda Red Recombineering of RP4 (Bryan)

Co-transformed DY331 with pUC307 (RP1) and recombinant vector containing Cm resistance cassette. Time constants 1.0 and 1.2 for two trials. Suspect glycerol stocks may contribute to poor time constant. Plated on selective media.

Since I expect poor transformation, innoculated ON culture of DY331 for next transformation experiment. DY331 will be rendered electrocompetent and immediately transformed to eliminate any problems that may be due to using a glycerol stock.


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