Team:Istanbul
From 2008.igem.org
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It has a huge quarternary structure consisting of 30% amylose and 70% amylopectin subunits. Compared to its counterpart in animals and bacteria, glycogen, starch is less branched. Nevertheless, this seemingly unimportant change makes it of paramount importance in a variety of industrial areas including but not limited to papermaking, oil exploration, and hydrogen production. More significantly, starch, as a carbon resource for secondary generation fuel, is promising for the future of bioenergy.</span></p> <p class=MsoBodyText style='text-align:justify;text-justify:newspaper; text-kashida-space:50%;text-align:justify;text-justify:newspaper;text-kashida-space: 50%'><span style='font-family:Arial;language:TR'><![if !supportEmptyParas]> <![endif]></span></p> <p class=MsoBodyText style='text-align:justify;text-justify:newspaper; text-kashida-space:50%;text-align:justify;text-justify:newspaper;text-kashida-space: 50%'><span style='font-family:Arial;language:TR'>Today’s research focuses on improving starch yield in plants by transfering ADPglucose pyrophosphorylase of </span><span style='font-family:Arial;font-style:italic;language:TR'>E.coli </span><span style='font-family:Arial;language:TR'>to plants. Since starch in plants can also be used as a food source, it occurred to us that it is a better idea to use recombinant </span><span style='font-family:Arial;font-style:italic;language:TR'>E.coli </span><span style='font-family:Arial;language:TR'>for purposes of bioenergy.</span></p> <p class=MsoBodyText><span style='language:EN'><![if !supportEmptyParas]> <![endif]></span></p> <p class=MsoBodyText style='text-align:justify;text-justify:newspaper; text-kashida-space:50%;text-align:justify;text-justify:newspaper;text-kashida-space: 50%'><span style='language:EN'>Thus, we aim to make recombinant </span><span style='font-family:Arial;font-style:italic;language:EN'>E.coli </span><span style='language:EN'>which produces starch instead of glycogen. In order to do this, we need to replace glycogen production genes in </span><span style='font-family:Arial;font-style:italic;language:EN'>E.coli </span><span style='language:EN'>by starch production genes of Zea maize (corn).</span></p> </div> <![if !vml]></td> </tr> </table> </span><![endif]><!--[if gte vml 1]><v:shape id="_x0000_s1057" type="#_x0000_t202" style='position:absolute;left:141.98pt;top:474.6pt;width:206.01pt;height:89.51pt; z-index:10;visibility:visible;mso-wrap-edited:f;mso-wrap-distance-left:2.88pt; mso-wrap-distance-top:2.88pt;mso-wrap-distance-right:2.88pt; mso-wrap-distance-bottom:2.88pt' filled="f" fillcolor="black [0]" stroked="f" strokecolor="#c99 [2]" strokeweight="0" o:cliptowrap="t"> <v:fill color2="white [7]"/> <v:stroke dashstyle="dash"> <o:left v:ext="view" color="black [0]" color2="white [7]"/> <o:top v:ext="view" color="black [0]" color2="white [7]"/> <o:right v:ext="view" color="black [0]" color2="white [7]"/> <o:bottom v:ext="view" color="black [0]" color2="white [7]"/> <o:column v:ext="view" color="black [0]" color2="white [7]"/> </v:stroke> <v:shadow color="#ccc [4]"/> <v:path insetpenok="f"/> <o:lock v:ext="edit" shapetype="t"/> <v:textbox style='mso-column-margin:5.7pt' inset="2.85pt,2.85pt,2.85pt,2.85pt"/> </v:shape><![endif]--><![if !vml]><span style='position:absolute;z-index:10; left:189px;top:633px;width:275px;height:120px'> <table cellpadding=0 cellspacing=0> <tr> <td width=275 height=120 valign=bottom style='vertical-align:top'><![endif]> <div v:shape="_x0000_s1057" style='padding:55.65pt 2.85pt 2.85pt 2.85pt' class=shape> <p class=MsoAddress><span style='language:EN'>Koç University</span></p> <p class=MsoAddress><span style='language:EN'>Rumelifeneri Yolu 34450 </span></p> <p class=MsoAddress><span style='language:EN'>Sarıyer Istanbul Turkey</span></p> </div> <![if !vml]></td> </tr> </table> </span><![endif]><!--[if gte vml 1]><v:shape id="_x0000_s1058" type="#_x0000_t202" style='position:absolute;left:142.23pt;top:446.31pt;width:205.68pt;height:28.29pt; z-index:11;visibility:visible;mso-wrap-edited:f;mso-wrap-distance-left:2.88pt; mso-wrap-distance-top:2.88pt;mso-wrap-distance-right:2.88pt; mso-wrap-distance-bottom:2.88pt' filled="f" fillcolor="black [0]" stroked="f" strokecolor="#c99 [2]" strokeweight="0" o:cliptowrap="t"> <v:fill color2="white [7]"/> <v:stroke dashstyle="dash"> <o:left v:ext="view" color="black [0]" color2="white [7]"/> <o:top v:ext="view" color="black [0]" color2="white [7]"/> <o:right v:ext="view" color="black [0]" color2="white [7]"/> <o:bottom v:ext="view" color="black [0]" color2="white [7]"/> <o:column v:ext="view" color="black [0]" color2="white [7]"/> </v:stroke> <v:shadow color="#ccc [4]"/> <v:path insetpenok="f"/> <o:lock v:ext="edit" shapetype="t"/> <v:textbox style='mso-column-margin:5.7pt' inset="2.85pt,2.85pt,2.85pt,2.85pt"/> </v:shape><![endif]--><![if !vml]><span style='position:absolute;z-index:11; 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