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Team:KULeuven/31 July 2008
From 2008.igem.org
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Contents |
Lab Work
Wet Lab
- A gel was run with the parts that were cut with XbaI during the night. the result was: B0015 cut, J23022 not cut, B0032 cut, J23032 cut. The positive parts were cut with EcoRI en those were again separated on gel, followed by purification. The following ligations were performed: J23100+B0032, R0084+B0032.
- Miniprep of the following parts was performed: R0011, P1010, C0062, I712022, C0060, R0062. We also nanodropped them and digested them with SpeI. The SpeI of La Roche is empty, so we used the enzyme of NEB. Because EcoRI doesn't cut very well in the buffer of SpeI (buffer 2), we began to purifie it by ethanol-precipitation. We put it overnight in -20°C.
- Competent TOP10 cells were transformed by heat shock with the ligation products that came out the overnight ligation.
Dry Lab
Primers for EnvZ::KmR mutation were made, will be ordered tomorrow.
Modeling
Wiki
- Worked on timer, keeping track on the time distance till the jamboree. Timer will be done tomorrow, after that the homepage will be taken care of.
- "The Project" tab has been taken care of, adding the details/mechanisms of the different parts. Tried to add figures and schemes for easier reading. Will be worked on whenever I further feel like it.
