Team:KULeuven/1 August 2008
From 2008.igem.org
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Lab Work
Wet Lab
- The ligated plasmids that were transformed into TOP10 cells yesterday, didn't give any colonies. We think that maybe the transfomation is the problem. So we wanted to use electroporation to transform the cells, but we ran out of competent cells.
- The primers to make GFP with LVA tag arrived today. So we did a PCR to amplify this part and to attach the prefix and suffix. GFP-LVA is now on its way to become a BioBrick (after ligation into a plasmid).
Dry Lab
Modeling
Part one of the mathematical analysis of the memory is done: stability of the two stationary states is proven. For next week: try to find the boundary between the initial states going to one of the two stationary states.