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Team:Valencia/Notebook/July
From 2008.igem.org
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July 1st
- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from Eduardo Rial.
- We calibrated our calorimeters with hot water.
July 2nd
- We prepared our first SD medium.
- We calibrated our calorimeters with hot water.
July 4th
- We prepared some plates for inoculum.
July 7th
- We prepared some inoculum for our experiment the following day.
July 8th
- We carried out the pilot experiment.
July 9th
- We characterized culture growth in our calorimeters with just one strain.
July 10th
- Trying to see if there is basal rate of UCP1...
July 11th
- It looks like our Saccharomyces cerevisiae strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.
July 12th
- We obtained the same results.
July 14th
- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.
July 15th
- We prepared inoculum.
July 16th
- Trying to find out which is the best moment for activating UCP1...
July 17th
- Our lab electricity went off during the night. How many experiments will have it ruined?
July 18th
- We prepared lots of plates with our SD medium...Unfortunately, we forgot to add glucose.
- We carried out the pilot experiment. We see weird oscillations.
July 20th
- We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.
July 21st
- We carried out the pilot experiment again. Weird results, we need to repeat it.
July 22nd
- We repaired our calorimeters. They were the reason for yesterday weird results.
July 23rd
- We repeated the pilot experiment.
July 24th
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...
July 25th
- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.
July 26th
- We obtain good results.
July 28th
- One of our calorimeters wasn't working. We repaired it and calibrated once again.
