Team:ESBS-Strasbourg/Transformation Heat shock

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  • ligation or 10 ng plasmid
  • add 50-100 µL of competent bacteria
  • take on ice for 30 min
  • heat shock at 42 °C for 2 min
  • add 300 µL LB and incubated at 37°C for 45 min
  • plate
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