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Team:BCCS-Bristol/Calendar-Notebook/4 July 2008
From 2008.igem.org
Swimming agar assay
We wanted to test different conditions for the swimming agar assay:
- different agar concentrations
- different incubation temperatures
- different inoculation depths
After an incubation overnight, we measured the diameter of the E. coli swarms. Surprisingly, we observed two swarm layers: The lower one has a bigger diameter than the upper.
| Agar (%) | Temperature ( o C) | Inoculation Depth | Number | JPG File | Inner Ring (cm) | Outer Ring (cm) |
| 0.3 | 30 | Deep | 1 | 4828 | 4.6 | 5.3 |
| 0.3 | 30 | Deep | 2 | 4827 | 4.6 | 5.3 |
| 0.3 | 30 | Deep | 3 | 4829 | 4.7 | 5.2 |
| 0.3 | 30 | Under Surface | 1 | 4830 | 4.6 | 5.2 |
| 0.3 | 30 | Under Surface | 2 | 4831 | 4.6 | 5.2 |
| 0.3 | 30 | Under Surface | 3 | 4832 | 4.6 | 5.1 |
| 0.3 | 30 | On Surface | 1 | 4833 | 2.9 | 3 |
| 0.3 | 30 | On Surface | 2 | 4834 | 3.8 | 4.4 |
| 0.3 | 30 | On Surface | 3 | 4835 | 4.4 | 4.9 |
| 0.3 | 37.5 | Under Surface | 1 | 4836 | 7.2 | 7.6 |
| 0.3 | 37.5 | Under Surface | 2 | 4837 | 7.4 | 7.7 |
| 0.3 | 37.5 | Under Surface | 3 | 4838 | 7.1 | 7.4 |
| 0.3 | 25 | Under Surface | 1 | 4840 | 2.8 | 3.5 |
| 0.3 | 25 | Under Surface | 2 | 4839 | 3 | 3.6 |
| 0.3 | 25 | Under Surface | 3 | 4841 | 3.1 | 3.7 |
| 0.2 | 30 | Under Surface | 1 | 4842 | 8.6 | 8.6 |
| 0.2 | 30 | Under Surface | 2 | 4843 | 8.6 | 8.6 |
| 0.2 | 30 | Under Surface | 3 | 4844 | 8.6 | 8.6 |
| 0.5 | 30 | Under Surface | 1 | 4847 | 2.3 | 2.3 |
| 0.5 | 30 | Under Surface | 2 | 4845 | 1.9 | 1.9 |
| 0.5 | 30 | Under Surface | 3 | 4846 | 1.5 | 1.5 |
