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Team:BCCS-Bristol/Calendar-Notebook/6 August 2008
From 2008.igem.org
BioBrick Transformation
Last Thursday, the primers [http://partsregistry.org/wiki/index.php/Part:BBa_G00100 VF2] and [http://partsregistry.org/Part:BBa_G00101 VR] arrived. These primers allow a confirmation of a succesful transformation of most BioBrick parts by means of PCR. The resulting fragment length depends on the specific BioBrick DNA.
Using VF2 and VR, a colony PCR are was conducted with all colonies that were obtained with chemical competent cells so far. Two colonies with the [http://partsregistry.org/Part:BBa_J63005 yeast ADH1 promoter] (No. 1 and 2, see left photo) and three colonies with a [http://partsregistry.org/Part:BBa_E0240 GFP generator] (No. 3-5, see left photo) were identified.
