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Team:KULeuven/6 August 2008
From 2008.igem.org
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Contents |
Lab Work
Wet Lab
Dry Lab
Check possibility of hybrid promotor to connect memory and timer (LuxI). This is to replace the antisense LuxI. Promotor would look something like this:
OperatorHSL_LuxR --- -35 box --- OperatorCII P22 --- -10 box
New modeling of the antisense LuxI and its target LuxI mRNA shows that this leaky repression is not a problem for the system. Still looking at the composite promoter though.
Literature
[http://jb.asm.org/cgi/content/full/190/13/4392?view=long&pmid=18083819 Lux regulatory region (pLux)]
Modeling
Wiki
Dr. Coli is everywhere, even at the URL.
Remarks
Discovery of the day. Two people beat us to the punch: [http://www.diagnosticinformationsystem.com/bios.html 1] and [http://www.sscofcny.com/doctors/coli.htm 2]
