Alberta NINT/5 August 2008
From 2008.igem.org
lab work (JD):
Collected some quantitative data of K102011/X+H and TAIn_X/X+H Found that perhaps a 1/100 dilution of insert might work better for ligation Carried out ligation of K102011 vector with TAIn_X inserts Transformed XL1-B Cells Plated on LB+kan35 Incubated at 37C Annealed new gates TA2, TA3, TA6 and TA7