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Team:University of Ottawa/5 August 2008
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Today in the Lab
Chris
- Inoculation control was contaminated; will reperform later today.
Digestion of Construct OA, OB and T Amp Product
- In order to increase the scope of our microwave digestion experiment, I added the following samples for comparison purposes.
| Sample | Condition |
| 1' | 5 sec. in microwave, 2 minutes on dry ice |
| 2' | 2x(5 sec. in/2 min. dry ice) |
| 3' | 10x(5 sec. in/2 min. dry ice) |
- Digestion was performed according to previous protocol, including denaturation at 80°C and addition of ligase+ ATP.
- Ran all products on a 1% gel; however, did not run digestion products as a control and had to redo the experiment.
Ligation Microwave Optimisation
- 1. Digestion
| Component | 1xvol.(μL) | 6xvol.(μL) |
| Buffer3 | 1 | 6 |
| BSA | 1 | 6 |
