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EPF-Lausanne/8 August 2008
From 2008.igem.org
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Cloning with the ligation products
The ligations done with the part which were not run through a gel yielded some colonies. We took 5 colonies from each of the two ligations which seemed to work (the one with diluted and the one with undiluted vector, as vector concentration is much higher than insert concentration. We plated them anew on Kan-Amp plates and also culture them overnight in LB with the correct antibiotic. The pUC19 control was given same treatment but with Amp resistance only.
Digestions
The digestion which had not worked was redone.
