Attended the Wisconsin iGEM team meeting
Re-evaluated our PCR reactions and primers
Decided to alter the temperatures and times of several steps.
Also we redesigned the primers used to clone out the srl operon.
Team Fungus:
Digested PCR product and pET28a vector
Ran 1% agrose gel of digestion and performed gel purification
-digested and undigested PCR product as well as digested vector showed correctly sized bands
-undigested vector showed up at about 3kb when compared to supercoil ladder and at 4kb when compared to linear 1kb ladder
Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit
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