Wisconsin: Lignin Project/11 July 2008

Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use.
Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit.
created chemically competent stocks of MG1655, JW3890 and RL357.
The stocks were frozen down and kept at -80C for future use.
Team Fungus
Researched the necessary environment for the disulfide bond formation needed in correct folding of LiP
Concluded that a oxidative cytoplasm was needed, thus the Novagen Origami strains were chosen as final transformation hosts.