Team:Mississippi State/9 July 2008

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  1. Make 500uM stock solution of Upstream and Downstream primers.
  2. Make working solution of primers: 1ul stock + 49ul ddH2O
  3. Prepare PCR mix (for each sample, BKM and RP): 39ul ddH2O + 5ul Buffer + 1ul dNTP + 1ul Upstream + 1ul Downstream + Template + Taq = 50ul total
  4. Run PCR
  5. Prepare 2nd PCR mix using 1ul of 1st PCR product
  6. Prepare and run 1% Agarose Gel w/ both 1st and 2nd PCR products
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