User:University of Washington/11 August 2008

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LuxR from AraC and TetR(Faifan)

-Miniprepped and sent sequence of ligated pSB1AC3 and promoter D29 (x3 of 25 degree Celsius ligation, x3 of 4 degree Celsius ligation)

-Grew overnight x3 from the single colony from QuikChange(trail#3) in Tsy + Amp

-Did Dpn1 reaction (continue from Ingrid's QuikChange#4), let sit from 3.5 hours, PCR purified and transformed into XL1-Blue (using 5ul DNA), grew on Amp plate.


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