Molecular Biology

We try by different way to assemble biobrick

We(Vincent) do a mistake

E1010 and F1610

We try last week to assemble two big parts because it is easy to purify it. We do ligation and transformation last week. The resultat was great all of the ten plates work. Monday, we decide to do a mini prep of these cells to purifiy DNA. Then we want to check the plasmide so we digest a small quantity of them with EcoRI and SpeI to prepare it to do a gel tomorrow.

by Gel

We try again to purifiy insert and vector by Gel.

We try to assemble :

R0040/B0034

R0071/B0034

and I1466/I1433

We successful have isolate from Gel : R0040, R0071 and B0034

We try to change strategy of isolate I1466 and I14033

I1466/I14033

I14033 is small. We let it in the vector. I1466 is big we use it to a insert. We cut with SpeI and PstI. We digest the plasmid and we are going to do tomorrow ligation.

Without gel, use PCR with biobrick primer

We want

http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase

EPF-Lausanne/11 August 2008

From 2008.igem.org

Contents

Molecular Biology

E1010 and F1610

by Gel

I1466/I14033

Without gel, use PCR with biobrick primer