EPF-Lausanne/13 August 2008

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Molecular biology

We do great mini-prep.


F1610/E1010

We do the gel of F1610 and E1010 from old mini-preps that we let digest all the night by SpeI and EcoRI. E1010 is great but F1610 doesn't work.

We don't know where come the mistake!!!

So we want to be sure that the part are correct and enzymes too. So we digest F1610 and E1010 from new mini-prep of today and we split experiences of digest in two EcoRI/SpeI and PstI/XbaI.

And we do different PCR by this way we don't use restriction enzyme. We do PCR with biobrick primer for E1010, F1610, F1610/E1010(assembled) and we do a positive control with DNA and Primers form Bart.

In finally we will run a gel with :

F1610 digest by EcoRI and SpeI

E1010 digest by EcoRI and SpeI

F1610 digest by PstI and XbaI

E1010 digest by PstI and XbaI

F1610 from PCR

E1010 from PCR

E1010/F1610 from PCR

Positiv control from PCR

E1010 (nothing, straight from mini-prep)

F1610 (nothing, straight from mini-prep)





mini-prep of R../B0034

PCR of minprep

I1466/I14033 gel purification

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