Team:Chiba/Calendar-Home/17 August 2008

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バイオブリックパーツおこし BBa_I73002 BBa_I73004 BBa_I73003 BBa_I763026

Elution :EB buffer 5μL -> Spin(15000rpm,3Min.)

Insert PCR


DNA template 1μL
Fwd primer(VF II) 5μL
Rev primer(VR) 5μL
Termo pol buffer 10μL
Vent pol 1μL
dH2O 68μL
==========================
Total:100μL


95℃(5min.) -> [95℃(30sec.) -> 53℃(1min.) -> 72℃(2min.)]x25cycle -> 72℃(10min.) -> store

Gel check

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