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Team:BCCS-Bristol/Protocols-Media
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Revision as of 19:44, 18 August 2008 by Tgorochowski (Talk | contribs)
Media
LB broth or agar (200 ml)
| Component | Concentration [%] | Amount for 200 ml | |
| Yeast Extract | 0.5 | 1 g | |
| Bactotryptone | 1 | 2 g | |
| NaCl | 1 | 2 g | |
| Agar | For plates 2 | For plates 4 g |
Swimming agar medium
| Component | Concentration [%] | Amount for 200 ml | |
| Bactotryptone | 1 | 2 g | |
| NaCl | 0.5 | 2 g | |
| Agar | various |
SOC medium
| Component | Concentration [%] | Amount for 95 (100) ml | |
| Bactotryptone | 2 | 2 g | |
| Bacto yeast extract | 0.5 | 0.5 g | |
| NaCl | 0.05 | 0.05 g |
Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized):
• 0.5 ml 2 M MgCl2
• 2 ml 1 M Glucose
