Team:The University of Alberta/20 August 2008

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Today

  • The retransformed Site Directed Mutagenesis: Lots o' growth on the transformation control = good!

Growth on the Mutagenesis control = good! Growth on the binary vector = very good! EXCEPT: There were no blue colonies on the Mutagenesis control! Bad? Not really. We forgot to add IPTG and X-gal! And it doesnt really matter because we had blue colonies last time and we transformed from the same reaction so they should be blue once we add the reagents!

So we have our mutated binary vector. To confirm that it is what we want, we are going to try digesting with NotI and see how many fragments we end up with. Shouldnt be more than one...

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