From 2008.igem.org
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lab work (JD):
Gel purification of TA2,TA3, TA6, TA7/B+N
lab work (SD):
Quantitated pSB1A3.1,.2, and .3
Digested pSB1A3 with EcoRI+PstI
Incubated for 2 hrs at 37 C
Ran on 2% agarose gel (110 V for 60 min)
Excised fragments for pSB1A3.1 and pSB1A3.3
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