Team:Paris/Modeling/f1

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F1a.jpg
In the exponential phase of growth, at the steady state, we have
TetReqtot.jpg

so, with

F1expr.jpg

that gives, after reducing the expression

F1exprredu.jpg

with the previous expression of [TetR]eq

F1exprreduf0.jpg

so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")

F1exprCalc.jpg


param signification unit value
[expr(pTet)] expression rate of
pTet with RBS E0032 		nM.s-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b) 		s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
βtet production rate of pTet with RBS E0032
β1 		nM.s-1
(Ktet/{coeftetR}ntet) activation constant of pTet
K18 		nMntet
ntet complexation order of pTet
of n18 		no dimension
KaTc complexation constant aTc-TetR
K17 		nMnaTc
naTc complexation order aTc-TetR
n17 		no dimension
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