Team:Paris/Modeling/f8

From 2008.igem.org

Revision as of 17:44, 21 August 2008 by Hugo (Talk | contribs)
F8DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F8expr.jpg

gives

F8FlhDC.jpg

and

F8FliA.jpg

and for calculated values of the TF,

F8FlhDCCalc.jpg

and

F8FliACalc.jpg



param signification unit value
[expr(pFlhB)] expression rate of
pFlhB with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β57 production rate of FlhDC-pFlgB with RBS E0032
β57
nM.s-1
(K51/{coefflhDC}n51) activation constant of FlhDC-pFlgB
K51
nMn51
n51 complexation order of FliA-pFlgB
n51
no dimension
β58 production rate of FliA-pFlgB with RBS E0032
β52
nM.s-1
(K52/{coeffliA}n52) activation constant of FliA-pFlgA
K52
nMn52
n52 complexation order of FliA-pFlgB
n52
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlhB.jpg