Team:Paris/Modeling/f6

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F6DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F6expr.jpg

gives

F6FlhDC.jpg

and

F6FliA.jpg

and for calculated values of the TF,

F6FlhDCCalc.jpg

and

F6FliACalc.jpg



param signification unit value
[expr(pFlgA)] expression rate of
pFlgA with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β55 production rate of FlhDC-pFlgA with RBS E0032
β55
nM.s-1
(K49/{coefflhDC}n49) activation constant of FlhDC-pFlgA
K49
nMn49
n49 complexation order of FlhDC-pFlgA
n49
no dimension
β56 production rate of FliA-pFlgA with RBS E0032
β56
nM.s-1
(K50/{coeffliA}n50) activation constant of FliA-pFlgA
K50
nMn50
n50 complexation order of FliA-pFlgA
n50
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlgA.jpg