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User:University of Washington/21 August 2008
From 2008.igem.org
LuxR from AraC and TetR(Faifan)
- nanodropped the gel purified DNA at 230 nm
| ng/ul | 260/280 | 260/230 | |
|---|---|---|---|
| BBa_Elowitz | 2.1 | 1.56 | 0.03 |
| LuxR | 5.8 | 1.98 | 0.06 |
| GFP | 5.5 | 1.41 | 0.02 |
| P1010(KAN) | 4.0 | 1.97 | 0.02 |
- ligation
- mix
| dH20(ul) | Buffer(ul) | P1010-Kan(ul) | BBa_Elowitz(ul) | GFP(ul) | LuxR(ul) | T4 ligase(ul) | |
|---|---|---|---|---|---|---|---|
| GFP | 1.36 | 2 | 5 | 3.42 | 7.22 | - | 1>/td> |
| LuxR | 1.28 | 2 | 5.0 | 3.42 | - | 7.3 | 1>/td> |
- 1 hr incubate at room temp
- 15 mins denature at 65 degree Celsius
- 20 mins filtration
- transformed 2ul DNA into XL1-Blue, inoculated on Kan plate
MG1655Z1(Faifan)
-None of the four cultures grew on Tet, Kan, Cam
-Only culture#3 didn't grow on Amp
- Streaked #3 to new Tsy plate
- grew overnight of #3 in Tsy
LuxR from pLac
--I-insert and R-vector parts were isolated from their gel fragments and ligated together.
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