User:University of Washington/28 August 2008
From 2008.igem.org
LuxR from AraC and TetR(Faifan)
- ligation
- mix
- incubate at room temp 45 mins
- denature at 65 degree Celsius 10 mins
- filtered 20 mins
- transformed 2ul DNA into 45 ul XL1-Blue
- inoculated on CAM plates
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