Beijing Normal/27 July 2008
From 2008.igem.org
We and Tsinghua team share some experience on how to do the transformation of ligation product. We generalize some key words: 1. Make sure the concentration of the palsmids from the biobrick is high enough. 2. Keep a loe temprature when enzyme digestion is performed 3. The transformation product should be incubated for more than 1h.