Template:Team:UC Berkeley/Notebook/MJV construction

From 2008.igem.org

Revision as of 22:59, 4 June 2008 by Madhvi (Talk | contribs)

Construction of <Cre! Basic Part K112100 

PCR mv001/mv002 on pBca9145-Bca1117    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112100
-----------------------------------------------
mv001	Forward Biobricking of <Cre!
ggataGAATTCgtgAGATCTtccaatttactgaccgtacac
mv002	Reverse Biobricking of <Cre!
cgttaGGATCCttaatcgccatcttccagcaggcg

Construction of Cre> Basic Part K112101 

PCR mv003/mv004 on pBca9145-Bca1117    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112101
-----------------------------------------------
mv003	Forward Biobricking of Cre>
ggataGAATTCgtgAGATCTatgtccaatttactgaccgtac
mv004	Reverse Biobricking of Cre>
cgttaGGATCCatcgccatcttccagcaggcg


Construction of <Cre> Basic Part K112102 

PCR mv001/mv004 on pBca9145-Bca1117    (1057bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112102
-----------------------------------------------
mv001	Forward Biobricking of <Cre!
ggataGAATTCgtgAGATCTtccaatttactgaccgtacac
mv004	Reverse Biobricking of Cre>
cgttaGGATCCatcgccatcttccagcaggcg
Retrieved from "http://2008.igem.org/Template:Team:UC_Berkeley/Notebook/MJV_construction"