Team:KULeuven/29 August 2008

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Contents

Lab Work

Wet Lab

Dry Lab

Had another good meeting today. Possibility was raised that adenosine methylation by the dam methylase is inhibiting our XbaI GATC restrictions. Checking it out. JM109 is probably dam+ so XbaI shouldn't cut, while for example JM110 and DM1 (Invitrogen) are dam-. If we want to use XbaI we'll need dam- cells.

Modeling

Nick continued his succesful work on multicellularity in MatLab. He even created a graph that looks like the blueprints of some modern building + created bacteria that could go back in time ;)

We were also able to show the relative effect of the filter by outputting some graphs in MatLab.

Wiki

All parameters on the wiki AND in the MatLab model have been checked and should be correct.

Remarks

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