From 2008.igem.org
PCR al0001/al0002 on pSB3C6-Bjh1501 (508bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112300
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al0001 Forward Biobricking of lambda lysozyme with start and stop codons
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002 Reverse Biobricking of lambda lysozyme with start and stop codons
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0003/al0002 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112301
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al0003 Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0002 Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0001/al0004 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112302
-----------------------------------------------
al0001 Forward Biobricking of lambda lysozyme with start codon and no start codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0004 Reverse Biobricking of lambda lysozyme with start codon and no start codon
CGttaGGATCCtacatcaatctctctgaccgttccgcc
PCR al0003/al0004 on pSB3C6-Bjh1501 (502bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112303
-----------------------------------------------
al0003 Forward Biobricking of lambda lysozyme with no start/stop codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0004 Reverse Biobricking of lambda lysozyme with no start/stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc
PCR al0005/al0002 on pSB3C6-Bjh1501 (512bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112304
-----------------------------------------------
al0005 Forward Biobricking of lambda lysozyme with spacer
CCATAGAATTCatgAGATCTacatatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002 Reverse Biobricking of lambda lysozyme with spacer
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0006/al0002 on pSB3C6-Bjh1501 (527bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112305
-----------------------------------------------
al0006 Forward Biobricking of lambda lysozyme with rbs site
CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
al0002 Reverse Biobricking of lambda lysozyme with rbs site
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0007/al0008 on pSB3C6-Bjh1502 (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112306
-----------------------------------------------
al0007 Forward Biobricking of lambda holin with start and stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctg
al0008 Reverse Biobricking of lambda holin with start and stop codon
CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0009/al0008 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
-----------------------------------------------
al0009 Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctg
al0008 Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0007/al0010 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308
-----------------------------------------------
al0007 Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctg
al0010 Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc
PCR al0009/al0010 on pSB3C6-Bjh1502 (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309
-----------------------------------------------
al0009 Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctg
al0010 Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc
PCR al0011/al0008 on pSB3C6-Bjh1502 (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
-----------------------------------------------
al0011 Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTacatatgccagaaaaacatgacctg
al0008 Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0012/al0008 on pSB3C6-Bjh1502 (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311
-----------------------------------------------
al0012 Forward Biobricking of lambda holin with rbs site
???
al0008 Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0013/al0014 on pSB3C6-Bjh1503 (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
-----------------------------------------------
al0013 Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0014 Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0015/al0014 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
-----------------------------------------------
al0015 Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0014 Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0013/al0016 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
-----------------------------------------------
al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0016 Reverse Biobricking of lambda antiholin with start codon and no stop codon
CGttaGGATCCttgatttctaccatcttctactccgg
PCR al0015/al0016 on pSB3C6-Bjh1503 (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
-----------------------------------------------
al0015 Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0016 Reverse Biobricking of lambda antiholin with no start and stop codons
???
PCR al0017/al0014 on pSB3C6-Bjh1503 (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
-----------------------------------------------
al0017 Forward Biobricking of lambda antiholin with spacer
al0014 Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0018/al0014 on pSB3C6-Bjh1503 (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
-----------------------------------------------
al0018 Forward Biobricking of lambda antiholin with rbs site
CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc
al0014 Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc
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