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From 2008.igem.org

At the steady-state, we have

and

so the expression

gives

and

and for calculated values of the TF,

and

param	signification	unit	value
[expr(pFlgA)]	expression rate of pFlgA with RBS E0032	nM.min-1	see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM?
γGFP	dilution-degradation rate of GFP(mut3b)	min-1	0.0198
[GFP]	GFP concentration at steady-state	nM	need for 20 + 20 measures and 5x5 measures for the SUM?
(fluorescence)	value of the observed fluorescence	au	need for 20 + 20 measures and 5x5 measures for the SUM?
conversion	conversion ration between fluorescence and concentration	nM.au-1	(1/79.429)

param	signification corresponding parameters in the equations	unit	value
β55	production rate of FlhDC-pFlgA with RBS E0032 β55	nM.min-1
(K49/{coefflhDC})	activation constant of FlhDC-pFlgA K49	nM
n49	complexation order of FlhDC-pFlgA n49	no dimension
β56	production rate of FliA-pFlgA with RBS E0032 β56	nM.min-1
(K50/{coeffliA})	activation constant of FliA-pFlgA K50	nM
n50	complexation order of FliA-pFlgA n50	no dimension

Then, if we have time, we want to verify the expected relation

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