Rensselaer/8 September 2008

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DNA purification digest 

       Fe(13ug)  Plasmid(11ug)

DNA 280ul 95ul XbaI 10 10 SprI 20 20 Buffer2 40 17.5 BSA 4 1.75 dH2O 46 30.75 

       ____      _____
      400 ul     175 ul

Insert checks digest 

              Fe-mRFP (~1ug)

DNA(30ug/ul) 33 MsCI(3U/ul) 6.7 EcoRI(2U/ul) 1 EcoBuff 5 BSA 0.5 dH2O 3.8 

              _____
              50 ul
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