1. PCR - AID for translation fusion

Primers:

AIDlNrH AIDpLinB

Template DNA: pTrc99A-AID

Annealing temperature: 55 °C

Annealing time: 60 sec

20 cycles

2. PCR - T7 RNA-polimerase for translation fusion

Primers:

T7lLinkB T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Annealing time: 4 minutes

20 cycles

3. PCR - T7 RNA-polimerase for transcription fusion

Primers:

T7lRBSHi T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Annealing time: 4 minutes

20 cycles