1. Digest of pMPM-T5 with NcoI and XbaI

2. Digest of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI

3. Clean-up of digested products from p.1 and p.2

4. Ligation of digested products from p.1 and p.2

5. Transformation of E.coli TOP10 with ligation product

6. Digest of pMPM-T5+AID with HindIII and SalI

7. Digest of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI

8. Clean-up of digested products from p.6 and p.7

9. Ligation of digested products from p.6 and p.7

10. Transformation of E.coli TOP10 with ligation product

11. Transformants plating on LB + tetracycline

<img src="http://openwetware.org/images/thumb/6/61/UW_A%2BT.jpg/350px-UW_A%2BT.jpg"/> <img src="http://openwetware.org/images/thumb/c/c7/UW_AT.jpg/350px-UW_AT.jpg"/>