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Team:University of Lethbridge/Notebook/Project1September
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September 10, 2008
Selina, Christa, Munima
Created 10 mL sterile 100 mM theophylline solution. Aliquotted into microfuge tubes and placed in cabinet in teaching lab.
Note: higher solubility in EtOH (see Merck index) but will only reach 100 mM in a super-saturated H20 solution (heat up to 50C directly before use).
September 11, 2008
Christa, Selina, Munima
Made motility media tubes (~20 blank, ~12 with 1 mM theophylline, ~10 with 0.25 mM theophylline, 1 with 50 mM theophylline). Attempts to make 'layered' motility media stab tubes was a complete disaster.
Labeled tubes and placed in teaching lab beside LB culture tubes - DO NOT MIX THESE UP!!!!
