Team:Warsaw/Calendar-Main/2 June 2008

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1. Transformation of E.coli TOP10 with pZC320
2. LB + ampicillin(30μg/mL) + X-gal + IPTG
3. PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Primers: AIDlNrH and T7pXbSal
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Annealing temperature: 55°C
Time: 4 minutes
20 cycles

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