Team:Warsaw/Calendar-Main/10 June 2008

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1. Plasmid isolation from previous day's colonies.
2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and SalI
3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and EcoRI
4. Control digest of pMPM-T5 + T7 RNA-polimerase with EcoRI
5. Control digest of pMPM-T5 + T7 RNA-polimerase with BamHI and XhoI
6. DNA electrophoresis of digestion products
7. Choice of proper colonies of transformants

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