Making Overnight Cultures from Frozen Glycerol Cell Stocks

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Preparing overnight cultures
  • Pippette 10mL LB into a red-capped culture tube or conical shake flask.
  • Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.)
  • Incubate at 37°C overnight.
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