Making Frozen Glycerol Cell Stocks From Overnight Cultures

From 2008.igem.org

Revision as of 15:17, 15 September 2008 by Riachalder (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Take 2mls of ON culture and pipette into a sterilised cryo tube.
Add 0.5ml of autoclaved 50% glycerol solution
Vortex briefly to mix
Store at -80˚C

Retrieved from "http://2008.igem.org/Making_Frozen_Glycerol_Cell_Stocks_From_Overnight_Cultures"