Team:Newcastle University/Conclusions

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Newcastle University

GOLD MEDAL WINNER 2008

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Results

Microscopy results of iGEMgfp upon 1% induction by subtilin

Microscopy results of iGEMcherry upon 1% induction by subtilin

Flow cytometry results for 1% induction by subtilin.

Flow cytometry results for 10% induction by subtilin.

To analyse the results of the wet lab transformations of the inserts into B. subtilis, we used two methods: microscopy and flow cytometry.

Microscopy work done on 08.09.08 showed a possible difference in the brightness of the iGEMgfp fluorescent cells (brighter in 10% subtilin-induced cells than in 0% subtilin-induced cells). However, there was little difference in the numbers of cells that fluoresced between the two cultures. There was no difference in either the number of fluorecent cells or their brightness between the 10% subtilin-induced and the 0% subtilin-induced iGEMcherry cells.

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