1. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB

2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose

3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB

4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG

5. Wait for 5 hours

6. OD measurment

7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + riphampicin)

PCR results of translational fusion for the A+AT construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.