Newcastle University Drylab/22 May 2008

From 2008.igem.org

Revision as of 20:27, 21 September 2008 by Mtaschuk (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Bugbuster-logo-red.png
Ncl uni logo.jpg


Newcastle University

GOLD MEDAL WINNER 2008

Home Team Original Aims Software Modelling Proof of Concept Brick Wet Lab Conclusions


Home >> Dry Lab >> Dry Lab Journal

May
MTWTFSS
      [http://2008.igem.org/Newcastle_University_Drylab/1_May_2008 1] [http://2008.igem.org/Newcastle_University_Drylab/2_May_2008 2] [http://2008.igem.org/Newcastle_University_Drylab/3_May_2008 3] [http://2008.igem.org/Newcastle_University_Drylab/4_May_2008 4]
[http://2008.igem.org/Newcastle_University_Drylab/5_May_2008 5] [http://2008.igem.org/Newcastle_University_Drylab/6_May_2008 6] [http://2008.igem.org/Newcastle_University_Drylab/7_May_2008 7] [http://2008.igem.org/Newcastle_University_Drylab/8_May_2008 8] [http://2008.igem.org/Newcastle_University_Drylab/9_May_2008 9] [http://2008.igem.org/Newcastle_University_Drylab/10_May_2008 10] [http://2008.igem.org/Newcastle_University_Drylab/11_May_2008 11]
[http://2008.igem.org/Newcastle_University_Drylab/12_May_2008 12] [http://2008.igem.org/Newcastle_University_Drylab/13_May_2008 13] [http://2008.igem.org/Newcastle_University_Drylab/14_May_2008 14] [http://2008.igem.org/Newcastle_University_Drylab/15_May_2008 15] [http://2008.igem.org/Newcastle_University_Drylab/16_May_2008 16] [http://2008.igem.org/Newcastle_University_Drylab/17_May_2008 17] [http://2008.igem.org/Newcastle_University_Drylab/18_May_2008 18]
[http://2008.igem.org/Newcastle_University_Drylab/19_May_2008 19] [http://2008.igem.org/Newcastle_University_Drylab/20_May_2008 20] [http://2008.igem.org/Newcastle_University_Drylab/21_May_2008 21] [http://2008.igem.org/Newcastle_University_Drylab/22_May_2008 22] [http://2008.igem.org/Newcastle_University_Drylab/23_May_2008 23] [http://2008.igem.org/Newcastle_University_Drylab/24_May_2008 24] [http://2008.igem.org/Newcastle_University_Drylab/25_May_2008 25]
[http://2008.igem.org/Newcastle_University_Drylab/26_May_2008 26] [http://2008.igem.org/Newcastle_University_Drylab/27_May_2008 27] [http://2008.igem.org/Newcastle_University_Drylab/28_May_2008 28] [http://2008.igem.org/Newcastle_University_Drylab/29_May_2008 29] [http://2008.igem.org/Newcastle_University_Drylab/30_May_2008 30] [http://2008.igem.org/Newcastle_University_Drylab/31_May_2008 31]
June
MTWTFSS
            [http://2008.igem.org/Newcastle_University_Drylab/1_June_2008 1]
[http://2008.igem.org/Newcastle_University_Drylab/2_June_2008 2] [http://2008.igem.org/Newcastle_University_Drylab/3_June_2008 3] [http://2008.igem.org/Newcastle_University_Drylab/4_June_2008 4] [http://2008.igem.org/Newcastle_University_Drylab/5_June_2008 5] [http://2008.igem.org/Newcastle_University_Drylab/6_June_2008 6] [http://2008.igem.org/Newcastle_University_Drylab/7_June_2008 7] [http://2008.igem.org/Newcastle_University_Drylab/8_June_2008 8]
[http://2008.igem.org/Newcastle_University_Drylab/9_June_2008 9] [http://2008.igem.org/Newcastle_University_Drylab/10_June_2008 10] [http://2008.igem.org/Newcastle_University_Drylab/11_June_2008 11] [http://2008.igem.org/Newcastle_University_Drylab/12_June_2008 12] [http://2008.igem.org/Newcastle_University_Drylab/13_June_2008 13] [http://2008.igem.org/Newcastle_University_Drylab/14_June_2008 14] [http://2008.igem.org/Newcastle_University_Drylab/15_June_2008 15]
[http://2008.igem.org/Newcastle_University_Drylab/16_June_2008 16] [http://2008.igem.org/Newcastle_University_Drylab/17_June_2008 17] [http://2008.igem.org/Newcastle_University_Drylab/18_June_2008 18] [http://2008.igem.org/Newcastle_University_Drylab/19_June_2008 19] [http://2008.igem.org/Newcastle_University_Drylab/20_June_2008 20] [http://2008.igem.org/Newcastle_University_Drylab/21_June_2008 21] [http://2008.igem.org/Newcastle_University_Drylab/22_June_2008 22]
[http://2008.igem.org/Newcastle_University_Drylab/23_June_2008 23] [http://2008.igem.org/Newcastle_University_Drylab/24_June_2008 24] [http://2008.igem.org/Newcastle_University_Drylab/25_June_2008 25] [http://2008.igem.org/Newcastle_University_Drylab/26_June_2008 26] [http://2008.igem.org/Newcastle_University_Drylab/27_June_2008 27] [http://2008.igem.org/Newcastle_University_Drylab/28_June_2008 28] [http://2008.igem.org/Newcastle_University_Drylab/29_June_2008 29]
[http://2008.igem.org/Newcastle_University_Drylab/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Newcastle_University_Drylab/1_July_2008 1] [http://2008.igem.org/Newcastle_University_Drylab/2_July_2008 2] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/3_July_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/4_July_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/5_July_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/6_July_2008&action=edit 6]
[http://2008.igem.org/Newcastle_University_Drylab/7_July_2008 7] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/8_July_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/9_July_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/10_July_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/11_July_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/12_July_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/13_July_2008&action=edit 13]
[http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/14_July_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/15_July_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/16_July_2008&action=edit 16] [http://2008.igem.org/Newcastle_University_Drylab/17_July_2008 17] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/18_July_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/19_July_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/20_July_2008&action=edit 20]
[http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/21_July_2008&action=edit 21] [http://2008.igem.org/Newcastle_University_Drylab/22_July_2008 22] [http://2008.igem.org/Newcastle_University_Drylab/23_July_2008 23] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/24_July_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/25_July_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/26_July_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/27_July_2008&action=edit 27]
[http://2008.igem.org/Newcastle_University_Drylab/28_July_2008 28] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/29_July_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/30_July_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/31_July_2008&action=edit 31]
August
MTWTFSS
        [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/1_August_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/2_August_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/3_August_2008&action=edit 3]
[http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/4_August_2008&action=edit 4] [http://2008.igem.org/Newcastle_University_Drylab/5_August_2008 5] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/6_August_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/7_August_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/8_August_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/9_August_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/10_August_2008&action=edit 10]
[http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/11_August_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/12_August_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/13_August_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/14_August_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/15_August_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/16_August_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/17_August_2008&action=edit 17]
[http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/18_August_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/19_August_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/20_August_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/21_August_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/22_August_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/24_August_2008&action=edit 24]
[http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/25_August_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/26_August_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/27_August_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/28_August_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/29_August_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Drylab/31_August_2008&action=edit 31]

22 May 2008

Mark

Began this morning by finishing off my java program which makes noisy data. The program now takes a file in containing the relevant perfect data, and produces some nice noisy data to prevent underfitting of the network. This is given in an output file. The exercise has been useful both for proof of concept, and helping me with my Java skills and has some good value for using neural network software. Have also decided to add to this week's agenda by adding a discussion on what programs to practice Java coding, providing that they are relevant to the topic.

Matt came in at 11 and helped me finish off my program. Note to self - there is a programmer in there somewhere, its just actually learning how to get the stuff down on paper that is the problem at the moment. Need more practice. Matt went through the svn system with everyone (including some non-iGEM intruders) and we used my system that we got running yesterday as an example of how the svn system actually works. It was developed for open source code sharing.

At 12 Marcus Kaiser came in and went through with each of us where we are with out projects. I gave him a working demo of my neural net and new java doc software, and oulined my plan for the coming weeks as well as talked about the Biological concept.

Just going on again to looking through the Sourceforge database for relevant programs and structures. Prior to the meeting at 2 30 we should also have all our biological concept stuff nailed down and ready to discuss.

We are also just about to do a bit of last minute Marketing brainstorming.

Had the meeting at 2. 30 and was very productive. Aims for the week are to look in greater depth at the proteins involved in the Listeria and Staphylococcus sytems, and to start writeing an evolutionary algorithm that maps input nodes to output nodes, and evaluates fitness based on the highest number of connections between those nodes.


Megan

Find out for sure whether B. subtilis has the PapR/PlcR system like B. cereus

The expression of various chromosomal genes encoding extracellular factors (i.e., phospholipases C, proteases, cell wall proteins, enterotoxins and hemolysins) is activated by a pleiotropic regulator, PlcR, that is specific to the B. cereus group. The activity of PlcR depends on the presence of PapR, a small signaling peptide that acts as a quorum-sensing effector. PapR is exported by the bacterial cell, processed, presumably as a pentapeptide, and then reimported into the cell, where it interacts with PlcR to facilitate its binding to its DNA targets. This activating mechanism is strain specific, and this specificity is determined by the first residue of the pentapeptide.

(Slamti et al., 2005)


Morgan

Thinking about the whitespace legend thing in more detail, I think I have some coordinates skewed, with an x and y switched or something. Going to look for that now.

Aha, it was indeed just the width and the height switched. Fixed and commiting now.

Kind of made a cursory README file for the project. I have old libraries in there that I really should delete, but there you are.

Neil said that the workbench should be able to infer connections between parts. I thought maybe that when a part was dropped, connections could be suggested, which the user can confirm or deny.


Nina

Retrieved from "http://2008.igem.org/Newcastle_University_Drylab/22_May_2008"