Team:Warsaw/Calendar-Main/24 June 2008

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1. PCR - translation fusion: AID + T7 RNA-polimerase Primers: AIDlNrH and T7pXbSal Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes two reapeats: 20 and 25 cycles 2. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase)



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