Team:Warsaw/Calendar-Main/23 May 2008

From 2008.igem.org

Revision as of 20:15, 22 September 2008 by MKrzyszton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



1. Digest of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI

2. Digest of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI

3. Clean-up of digested products from p.1 and p.2

4. Digest of pMPM-T5+AID with HindIII and SalI

5. Digest of pMPM-T5 with NcoI and XbaI

6. Gel-electrophresis and gel-out of proper bands.

7. Ligation of purified products from p.1 and p.4

8. Transformation of E.coli TOP10 with ligation product

9. Ligation of digested products from p.4 and p.5

10. Transformation of E.coli TOP10 with ligation product

11. Transformants plating on LB + tetracycline


http://openwetware.org/images/thumb/6/61/UW_A%2BT.jpg/350px-UW_A%2BT.jpg http://openwetware.org/images/thumb/c/c7/UW_AT.jpg/350px-UW_AT.jpg