Illinois/31 July 2008

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Receptor Tyrosie Kinase Group

PCR to asemble fragments from yesterday.

tube	1	2	3	4
template	0.5ul of both heavy and light chains
primers	1ul of appropriate forward and reverse primer
MgCl2	0ul	0ul	3ul	3ul
master mix	20ul
H20	to 50ul total volume

PCR program: 1. 94 degrees 5 min 2. 94 degrees 1 min 3. 50 degrees 1 min 4. 72 degrees 1 min 5. GOTO 2. 29 cycles 6. HOLD at 4 degrees

Products run on a 1.5% agarose gel, no products of 700-800bp.