Preparation of constructs with OmpA protein fusions
1. PCR on OmpA_linker and linker_alpha with ompaL_N and alphaP_XB primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees)
2. PCR on OmpA_linker and linker_omega with ompaL_N and omegaP_EPB primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees)
3. Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega
4. Overnight digestation of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI
5. Overnight digestation of pET15b plasmid with NdeI and BamHI, dephosphorylation with CIAP